Biotinylated SARS-CoV-2 Spike Trimer Protein (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N), His, Avitag™ (MALS verified) | SPN-C82Ec-200ug

Biotinylated SARS-CoV-2 Spike Trimer Protein (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N), His, Avitag™ (MALS verified) | SPN-C82Ec-200ug| Gentaur Distribution US, UK & Europe

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source: Biotinylated SARS-CoV-2 Spike Trimer, His, Avitag (SPN-C82Ec) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N) were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; other names: 21A/S:478K). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Format: Powder.

Tag: C-10×His & C-Avi.

Expression System: HEK293.

Expression Region: Val 16 - Pro 1213.

Conjugate: Biotin-labeled.

Molecular Weight: 139.5 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.5 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: PBS.

Storage Conditions: -20℃.

Shipping Conditions: RT