Description
Biotinylated SARS-CoV-2 Spike Trimer Protein (P26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H, T1027I, D1118H), His, Avitag™ (MALS verified) | SPN-C82Ed-200ug| Gentaur Distribution US, UK & Europe
It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source: Biotinylated SARS-CoV-2 Spike Trimer, His, Avitag (SPN-C82Ed) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (P26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H, T1027I, D1118H) were identified in the SARS-CoV-2 variant B.1.620, which was found to circulate in Europe and Central Africa. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Format: Powder.
Tag: C-10×His & C-Avi.
Expression System: HEK293.
Expression Region: Val 16 - Pro 1213.
Conjugate: Biotin-labeled.
Molecular Weight: 139.1 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.1 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: PBS.
Storage Conditions: -20℃.
Shipping Conditions: RT
