Description
Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) trimer, His, Avitag™ (MALS verified) | SPN-C82E6-200ug| Gentaur Distribution US, UK & Europe
It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source: Biotinylated SARS-CoV-2 S protein trimer, His, Avitag (SPN-C82E6) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The L18F/ T20N/ P26S/ D138Y/ R190S/ K417T/ E484K/ N501Y/ D614G/ H655Y/ T1027I/ V1176F) mutations were identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3).
Format: Powder.
Tag: C-10×His & C-Avi.
Expression System: HEK293.
Expression Region: Val 16 - Pro 1213.
Conjugate: Biotin-labeled.
Molecular Weight: 139.8 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: PBS.
Storage Conditions: -20℃.
Shipping Conditions: RT
