Biotinylated SARS-CoV-2 S protein, His, Avitag™, Super stable trimer (MALS verified) | SPN-C82E9-25ug

Biotinylated SARS-CoV-2 S protein, His, Avitag™, Super stable trimer (MALS verified) | SPN-C82E9-25ug| Gentaur Distribution US, UK & Europe

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source: Biotinylated SARS-CoV-2 S protein, His, Avitag, Super stable trimer (SPN-C82E9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Format: Powder.

Tag: C-10×His & C-Avi.

Expression System: HEK293.

Expression Region: Val 16 - Pro 1213.

Conjugate: Biotin-labeled.

Molecular Weight: 139.7 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.7 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: PBS.

Storage Conditions: -20℃.

Shipping Conditions: RT