Description
Biotinylated Mouse IL-27 Protein, His, Avitag™ | IL7-M82E4-25ug| Gentaur Distribution US, UK & Europe
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV) -induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.
Source: Biotinylated Mouse IL-27 Protein, His, Avitag (IL7-M82E4) is expressed from human 293 cells (HEK293). It contains AA Tyr 19 - Pro 228 (IL-27B) & Phe 29 - Ser 234 (IL-27A) (Accession # O35228-1 (IL-27B) & Q8K3I6-1 (IL-27A) ).
Format: Powder.
Tag: C-10×His & C-Avi.
Expression System: HEK293.
Expression Region: Tyr 19 - Pro 228 (IL-27B) & Phe 29 - Ser 234 (IL-27A).
Conjugate: Biotin-labeled.
Molecular Weight: 51.6 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 51.6 kDa. The protein migrates as 55 kDa & 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: 20 mM MOPS, 150 mM NaCl, 0.05% CHAPS, 1mM EDTA, pH7.0.
Storage Conditions: -20℃.
Shipping Conditions: RT
