Description
Biotinylated Human PD-1 / PDCD1 Protein, Fc, Avitag™, His Tag (MALS verified) | PD1-H82F4-200ug| Gentaur Distribution US, UK & Europe
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
Source: Biotinylated Human PD-1, Fc, Avitag, His Tag (PD1-H82F4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Format: Powder.
Tag: C-Fc & C-Avi & C-10×His.
Expression System: HEK293.
Expression Region: Leu 25 - Gln 167.
Conjugate: Biotin-labeled.
Molecular Weight: 45.5 kDa.
Characteristics: This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc tag and polyhistidine tag. The protein has a calculated MW of 45.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition, and 115-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
