Description
Biotinylated Human Osteopontin (OPN) Protein, His, Avitag™ | OPN-H82E8-25ug| Gentaur Distribution US, UK & Europe
Osteopontin (OPN) is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Source: Biotinylated Human Osteopontin, His, Avitag (OPN-H82E8) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # P10451-5).
Format: Powder.
Tag: C-10×His & C-Avi.
Expression System: HEK293.
Expression Region: Ile 17 - Asn 300.
Conjugate: Biotin-labeled.
Molecular Weight: 35.7 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 35.7 kDa. The protein migrates as 16 kDa, 23 kDa, 28 kDa and 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic processing of several intracellular enzymes.
Purity: 80%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
