Description
Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His, Avitag™&Tag Free | IT5-H82Wa-25ug| Gentaur Distribution US, UK & Europe
Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.
Source: Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His, Avitag&Tag Free (IT5-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # P06756-1 (ITGAV) & P18084-1 (ITGB5) ).
Format: Powder.
Tag: C-10×His & C-Avi | Native.
Expression System: HEK293.
Expression Region: Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5).
Conjugate: Biotin-labeled.
Molecular Weight: 114.7 kDa (ITGAV) & 81.9 kDa (ITGB5).
Characteristics: Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGAV) and 90-98 kDa (ITGB5) respectively due to glycosylation.
Purity: 95%.
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
