Biotinylated Human IL-2 R alpha / CD25 Protein, Fc, Avitag™ (MALS verified) | ILA-H82F9-25ug

Biotinylated Human IL-2 R alpha / CD25 Protein, Fc, Avitag™ (MALS verified) | ILA-H82F9-25ug| Gentaur Distribution US, UK & Europe

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

Source: Biotinylated Human IL-2 R alpha, Fc, Avitag (ILA-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # P01589-1).

Format: Powder.

Tag: C-Fc & C-Avi.

Expression System: HEK293.

Expression Region: Glu 22 - Cys 213.

Conjugate: Biotin-labeled.

Molecular Weight: 49.9 kDa.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 49.9 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.

Storage Conditions: -20℃.

Shipping Conditions: RT