Description
Biotinylated Human CD73 / NT5E Protein, His, Avitag™ | CD3-H82E3-25ug| Gentaur Distribution US, UK & Europe
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Source: Biotinylated Human CD73, His, Avitag (CD3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).
Format: Powder.
Tag: C-10×His & C-Avi.
Expression System: HEK293.
Expression Region: Trp 27 - Ser 549.
Conjugate: Biotin-labeled.
Molecular Weight: 61.7 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 61.7 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 90%.
Buffer: 20 mM Tris, 120 mM NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
