Description
Biotinylated ENPP3 protein, His, Avitag™ (active enzyme) | EN3-H82E5-200ug| Gentaur Distribution US, UK & Europe
The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).
Source: Biotinylated ENPP3, His, Avitag (EN3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Leu 48 - Ile 875 (Accession # O14638-1).
Format: Powder.
Tag: N-Avi & N-10×His.
Expression System: HEK293.
Expression Region: Leu 48 - Ile 875.
Conjugate: Biotin-labeled.
Molecular Weight: 98.6 kDa.
Characteristics: This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 98.6 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 90%.
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
