Biotin / Vitamin B7 / Vitamin H Antibody | V3649IHC-7ML

Biotin / Vitamin B7 / Vitamin H Antibody | V3649IHC-7ML | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Format: Purified

Clone: BTN/2032R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Biotin

Application: IHC-P

Application Details: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Note: The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.

Storage: Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: target location