Biotin / Vitamin B7 / Vitamin H Antibody | V3649-100UG

Biotin / Vitamin B7 / Vitamin H Antibody | V3649-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: BTN/2032R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Biotin

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.

Storage: Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: target location