Description
Biotin / Vitamin B7 / Vitamin H Antibody | V3649-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: BTN/2032R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Biotin
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note: The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
Storage: Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: target location
