BHK-21 [C-13] Cell Line | CL-0034

BHK-21 [C-13] Cell Line | CL-0034 | Gentaur US, UK & Europe Disrtribition

Media: CM-0034

Organism: Mesocricetus auRatus, Hamster, Syrian golden

Application: The World Organization for Animal Health(OIE) uses these cells for routine diagnosis of rabies. This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs.

Background: This cell line is reverse transcriptase negative.

Age: 1 day

Tissue: Kidney

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days