Description
Bcl-2 Antibody | V8937-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rBCL2/6418
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG2a, kappa
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Immunogen: Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Storage: Aliquot the recombinant Bcl-2 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Cytoplasm, nuclear membrane
