Bcl-2 Antibody | V3546-100UG

Bcl-2 Antibody | V3546-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: rBCL2/782

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: FACS, IF, WB, IHC-P

Application Details: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: Optimal dilution of the recombinant Bcl2 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein G affinity chromatography

Description: Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Bcl2 antibody.

Storage: Store the recombinant Bcl2 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cytoplasmic, membrane