Description
Basic Cytokeratin Antibody | V3812SAF-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free
Format: Purified
Clone: KRTH/2147R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human, Mouse, Rat
Application: FACS, IF, WB, IHC-P
Application Details: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2 ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note: Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Purity: Protein A affinity chromatography
Description: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Storage: Store the recombinant Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic
