Description
BALB/3T3 clone A31 Cell Line | CL-0477 | Gentaur US, UK & Europe Disrtribition
Media: CM-0477
Organism: Mus musculus, Mouse
Application: N/A
Background: The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus, SV40, and murine sarcoma virusTested and found negative for ectromelia virus(mousepox).
Age: Embryo; 14 to 17 days gestation
Tissue: Embryo
Morphology: Fibroblast
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: No, in immunosuppressed mice; Yes, in semisolid medium.
Duration: N/A
Lead Time: 3-5 days
