Description
AtT-20 Cell Line | CL-0028 | Gentaur US, UK & Europe Disrtribition
Media: CM-0028
Organism: Mus musculus, Mouse
Application: Transfection host.
Background: Tested and found negative for ectromelia virus(mousepox).
Age: N/A
Tissue: Pituitary
Morphology: Small rounded cells
Growth Properties: Mixed, Adherent And Suspension
Doubling Time: N/A
Biosafty: 1
Medium: Ham's F-12K(PM150910)+15% HS+2.5% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 1:2 to 1:4
Renewal: 2 to 3 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
