AsPC-1 Cell Line | CL-0027

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SKU:
575-CL-0027
Weight:
1.00 KGS
€1,432.00
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Description

AsPC-1 Cell Line | CL-0027 | Gentaur US, UK & Europe Disrtribition

Media: CM-0027

Organism: Homo sapiens, Human

Application: Transfection host.

Background: The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.

Age: 62 years

Tissue: Pancreas, derived from metastatic site: ascites

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:6

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days

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