Anti-5-mC monoclonal antibody 33D3 | 25207

Anti-5-mC monoclonal antibody 33D3 | 25207 | Gentaur US, UK & Europe Disrtribition

Category: Epigenetics/Antibody

Application: MeDIP (1 - 2 µg/IP)
ELISA (1:100)
DB (1:250)
IF (1:500)
FISH (1:500)
Southern blot (1:200)

Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

Description: Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumin. The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. It has been validated for Methylated DNA Immunoprecipitation (MeDIP-seq, MeDIP-on-chip), Immunofluorescence, Flow Cytometry, Dot blot & ELISA.

Product Type: Antibody

Shippement Condition: -20°C or -80°C