Description
Anti-5-hmC polyclonal antibody (rabbit) | 25205 | Gentaur US, UK & Europe Disrtribition
Category: Epigenetics/Antibody
Application: hMeDIP (2.5 µg/IP)
ELISA (1:500)
DB (1:200)
Background: 5-hydroxymethylcytosine (5-hmC) results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. 5-hmC bases were recently discovered in mammalian DNA, in Purkinje neurons, in granule cells and embryonic stem cells where theyare present at high levels (up to 0.6% of total nucleotides in Purkinje cells). Recent reports indicate that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests 5-hydroxymethylcytosine may represent a new and unique pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. The most commonly used methodologies (e.g. enzymatic approaches, bisulfite sequencing) do not distinguish 5mc from 5-hmC. The development of specific antibodies appears to be the most powerful way to distinguish and specifically enrich for 5-mC and 5-hmC sequences.
Description: Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine (5-hmC) conjugated to KLH
Product Type: Antibody
Shippement Condition: -20°C or -80°C
