AN3 CA cell line | CL-0505

AN3 CA cell line | CL-0505 | Gentaur US, UK & Europe Disrtribition

Media: CM-0505

Organism: Homo sapiens, human

Application: N/A

Background: This cell line was established in 1962 from the lymph node metastasis resected from a 55-year-old white woman with an endometrial adenocarcinoma (epithelial undifferentiated small cell type) presenting with acanthosis nigricans; forms heterotransplantable tumors.

Age: Female,55 years

Tissue: Uterus; endometrium

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~55 hours

Biosafty: 1

Medium: MEM(PM150467)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:5

Renewal: Every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days