Description
AML12 cell line | CL-0602 | Gentaur US, UK & Europe Disrtribition
Media: CM-0602
Organism: Mus musculus, mouse
Application: N/A
Background: The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.
Age: Male
Tissue: Liver
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~37 hours
Biosafty: 1
Medium: DMEM/F12(PM150312)+10% FBS+10 µg/ml Insulin+5.5 µg/ml Transferrin+5 ng/ml Selenium+40 ng/ml Dexamethasone+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
