AML12 cell line | CL-0602

AML12 cell line | CL-0602 | Gentaur US, UK & Europe Disrtribition

Media: CM-0602

Organism: Mus musculus, mouse

Application: N/A

Background: The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.

Age: Male

Tissue: Liver

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~37 hours

Biosafty: 1

Medium: DMEM/F12(PM150312)+10% FBS+10 µg/ml Insulin+5.5 µg/ml Transferrin+5 ng/ml Selenium+40 ng/ml Dexamethasone+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:5

Renewal: Every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days