AKT1 Antibody | V8442-20UG

AKT1 Antibody | V8442-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: AKT1/3898R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG

Species Reactivity: Human

Application: WB, ELISA

Application Details: Western blot: 1-2ug/ml,ELISA: order Ab without BSA for coating

Application Note: Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.

Purity: Protein A affinity chromatography

Description: Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Immunogen: A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.

Storage: Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Nuclear, cytoplasmic, cell surface