Description
AKT Antibody / PH domain | R20335-0.1ML | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Format: Purified
Clone: RM316
Host Animal: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Species Reactivity: Human
Application: WB, IHC-P
Buffer: N/A
Limitation: This recombinant AKT antibody is available for research use only.
Purity: Protein A purified from animal origin-free supernatant
Description: Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.
Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. This domain binds to phosphoinositides with high affinity. In the case of the PH domain of Akt, it binds either PIP3 (phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3) or PIP2 (phosphatidylinositol (3,4)-bisphosphate, PtdIns(3,4)P2).[9] This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. For example, PI 3-kinases may be activated by a G protein coupled receptor or receptor tyrosine kinase such as the insulin receptor. Once activated, PI 3-kinase phosphorylates PIP2 to form PIP3. [Wiki]
Immunogen: A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the recombinant AKT antibody.
Storage: Store the recombinant AKT antibody at -20 °C.
