Description
AGS Cell Line | CL-0022 | Gentaur US, UK & Europe Disrtribition
Media: CM-0022
Organism: Homo sapiens, Human
Application: Transfection host.
Background: The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection . Subsequently, AGS has been determined to be infected with Parainfluenza type 5(PIV5 formerly known as SV5).
Age: 54 years
Tissue: Stomach
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2
Medium: Ham's F-12(PM150810)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:6
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in athymic BALB/c mice.
Duration: N/A
Lead Time: 3-5 days
