Description
Actin Antibody / Muscle Specific | V8843-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rMSA/953
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: WB, IHC-P
Application Details: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-2ug/ml
Application Note: Optimal dilution of the Muscle Actin antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Immunogen: Recombinant protein was used as the immunogen for the Muscle Actin antibody.
Storage: Aliquot the Muscle Actin antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Cytoplasm
