ABO Antibody (Blood Group Antigen A) | V2549SAF-100UG

ABO Antibody (Blood Group Antigen A) | V2549SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: HE-14

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: IH, IF

Buffer: N/A

Limitation: This ABO antibody is available for research use only.

Purity: PEG precipitation

Description: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 mAb was used.

Immunogen: A mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.

Storage: Store the ABO antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).