Description
A-673 cell line | CL-0017 | Gentaur US, UK & Europe Disrtribition
Media: CM-0017
Organism: Homo sapiens, Human
Application: N/A
Background: This cell line was established from a 15-year-old female patient with a primary rhabdomyosarcoma. The cells can form colonies on soft agar and tumours in antithymocyte serum treated mice. This cell line contains more than four marker chromosomes, one extra F chromosome and two abnormal chromosomes.
Age: female, 15 years
Tissue: Muscle; Rhabdomyoma Sarcomatosum
Morphology: Polygonal
Growth Properties: Adherent
Doubling Time: ~28 hours
Biosafty: 1
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:4
Renewal: 2 to 3 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
