Description
A-431 Cell Line | CL-0015 | Gentaur US, UK & Europe Disrtribition
Media: CM-0015
Organism: Homo sapiens, Human
Application: Transfection host.
Background: The A-431 cell line was established by D.J. Giard in 1973.
Age: 85 years
Tissue: Skin, epidermis
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~80-100 hours
Biosafty: 1
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:6
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, forms rapidly growing subcutaneous tumors in immunosuppressed mice and colonies in soft agar.
Duration: N/A
Lead Time: 3-5 days
