786-O [786-0] Cell Line | CL-0010

786-O [786-0] Cell Line | CL-0010 | Gentaur US, UK & Europe Disrtribition

Media: CM-0010

Organism: Homo sapiens, Human

Application: Transfection host.

Background: The cells display both microvilli and desmosomes, and can be grown in soft agar.(PubMed: 2835539)The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors.The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons.

Age: 58 years

Tissue: Kidney

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~24-45 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:6

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in immunosuppressed hamsters.

Duration: N/A

Lead Time: 3-5 days