6T-CEM Cell Line | CL-0008

6T-CEM Cell Line | CL-0008 | Gentaur US, UK & Europe Disrtribition

Media: CM-0008

Organism: Homo sapiens, Human

Application: N/A

Background: This is a 6-thioguanine resistant variant of CCRF-CEM.It is deficient in Hypoxanthine phosphoribosyl transferase(HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.This line may be used as a fusion partner for making human T cell hybridomas.suspensionimmunosuppressive factorCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2×10 exp5 cells/ml and maintain between 1×10 exp5 and 1×10 exp6 cells/ml.Handle as potentially biohazardous material under at least Biosafety Level 2 containment.

Age: N/A

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 2

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days