Description
3T6-Swiss albino cell line | CL-0285 | Gentaur US, UK & Europe Disrtribition
Media: CM-0285
Organism: Mus musculus, Mouse
Application: N/A
Background: This cell line is derived from Swiss mouse embryos and secretes collagen and hyaluronic acid. This line is not contact inhibited. This cell line was tested for being not infected by ectromelia virus (mousepox).
Age: embryo
Tissue: Embryo; Fibroblast
Morphology: Fibroblast-Like
Growth Properties: Adherent
Doubling Time: ~15 hours
Biosafty: 1
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
