3T6-Swiss albino cell line | CL-0285

3T6-Swiss albino cell line | CL-0285 | Gentaur US, UK & Europe Disrtribition

Media: CM-0285

Organism: Mus musculus, Mouse

Application: N/A

Background: This cell line is derived from Swiss mouse embryos and secretes collagen and hyaluronic acid. This line is not contact inhibited. This cell line was tested for being not infected by ectromelia virus (mousepox).

Age: embryo

Tissue: Embryo; Fibroblast

Morphology: Fibroblast-Like

Growth Properties: Adherent

Doubling Time: ~15 hours

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days