Description
3T3-Swiss albino Cell Line | CL-0536 | Gentaur US, UK & Europe Disrtribition
Media: CM-0536
Organism: Mus Musculus, Mouse
Application: N/A
Background: Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture.Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition.Cells have now lost their contact inhibition.
Age: Embryo
Tissue: Embryo
Morphology: Fibroblast
Growth Properties: Adherent
Doubling Time: ~40 hours
Biosafty: 1
Medium: DMEM (PM150210)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: 1:2 to 1:4
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+50% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
