3T3-L1 Cell Line | CL-0006

3T3-L1 Cell Line | CL-0006 | Gentaur US, UK & Europe Disrtribition

Media: CM-0006

Organism: Mus musculus, Mouse

Application: Transfection host.

Background: The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation. Tested and found negative for ectromelia virus(mousepox).

Age: Embryo

Tissue: Embryo

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% CS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:5

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Effects: N/A

Duration: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum.

Lead Time: 3-5 days