Description
3T3-L1 Cell Line | CL-0006 | Gentaur US, UK & Europe Disrtribition
Media: CM-0006
Organism: Mus musculus, Mouse
Application: Transfection host.
Background: The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation. Tested and found negative for ectromelia virus(mousepox).
Age: Embryo
Tissue: Embryo
Morphology: Fibroblast
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10% CS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:5
Renewal: 2 to 3 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum.
Lead Time: 3-5 days
