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NGS Reagent

NGS Reagent

Next-generation sequencing (NGS) reagents 

Overview of chemical structure mapping followed by next-generation sequencing. Reagent molecules preferentially react with unconstrained nucleotides to modify them. Reverse transcriptase (RT) traverses the RNA and drops off upon encountering the first modification. RT may occasionally drop off prior to the modification in what is termed natural drop off. Sequencing of the resulting cDNA fragments reveals the sites of modification. When RT starts at a single predetermined primer binding site, one can control two parameters: average degree of modification, which depends on reagent concentration and reaction duration, and number of sequenced fragments, which depends on choice of sequencing coverage. Stochasticity in the composition of sequencing readouts arises from randomness in modification patterns, transcription termination events, and fragment sequencing.

 


Next-generation sequencing (NGS) reagents are essential elements used in the process of DNA sequencing. NGS has revolutionized genomics research by enabling rapid and cost-effective sequencing of entire genomes, transcriptomes, and other genetic materials. These reagents typically include:

Library Preparation Kits:

These kits are used to prepare DNA or RNA samples for sequencing. They include enzymes, adapters, primers, and other necessary components to fragment DNA, add adapters, and amplify the DNA fragments for sequencing.

 

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Sequencing Kits:

These kits contain the necessary reagents for the actual sequencing reaction, including nucleotides (A, T, C, G), polymerases, and buffers. They are tailored to specific sequencing platforms such as Illumina, Ion Torrent, PacBio, or Oxford Nanopore.

Indexing Primers and Barcodes:

Used in multiplexed sequencing to label individual samples, allowing them to be pooled together and sequenced simultaneously.

Quality Control Reagents:

Reagents for assessing the quality and quantity of DNA or RNA samples before sequencing, such as qPCR kits, gel electrophoresis reagents, and fluorometric assays.

Clean-up and Purification Kits:

Reagents for purifying and concentrating DNA or RNA samples before sequencing, including magnetic bead-based purification kits or column-based purification kits.

Data Analysis Tools:

While not strictly reagents, these software tools are often provided along with NGS kits to analyze the raw sequencing data and interpret the results.

 

 

 

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  • Hieff  NGS® OnePot Pro DNA Library Prep Kit for Illumina

    Hieff  NGS® OnePot Pro DNA Library Prep Kit for Illumina | 12205ES97

    €8,112.00
    Hieff  NGS® OnePot Pro DNA Library Prep Kit for Illumina | 12205ES97| Gentaur Distribution US, UK & Europe Hieff NGSTM OnePot Pro DNA Library Prep Kit for Illumina is a new generation enzymatic fragmentation-based library prep kit professionally...
    €8,112.00
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